Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay
نویسندگان
چکیده
منابع مشابه
Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes.
The use of gold nanoparticles (AuNPs) as labeling carriers in combination with the enzymatic activity of the horseradish peroxidase (HRP) in order to achieve an improved optical lateral flow immunoassay (LFIA) performance is presented here. Briefly in a LFIA with an immune-sandwich format AuNPs are functionalized with a detection antibody already modified with HRP, obtaining an 'enhanced' label...
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The infrared emissivity of Au and silica-coated Au nanoparticles (Au NPs) deposited on indium tin oxide substrates was investigated. NPs were irradiated with laser light at a frequency close to the Au plasmon resonance band, and the blackbody radiation emitted as a result was monitored with an IR camera equipped with an InAs array detector. The differences in temperature before and after laser ...
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EXPERIMENTAL SECTION Materials and reagents Tetrabutyl titanate (TBOT), sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS) and phosphoric acid (H3PO4) were from Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). Human IgG (HIgG) (Ag), rabbit anti-human IgG (Ab1), and goat anti-human IgG (Ab2) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China)....
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Enzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine (polyA)-Au interactions. In a SNAzyme, AuNPs serve as the metal c...
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ژورنال
عنوان ژورنال: Sensors
سال: 2020
ISSN: 1424-8220
DOI: 10.3390/s20123608